To identify specificComponents of the insulin signaling pathway that could potentially be involved in the pathogenesis of PCOS in NAFLD patients, the expression levels of ten target genes related to insulin regulation were investigated: FBP1, NIN, PPP1CA, LDLR, SREBF1, ACACA, RAF1, ACOX1, SORBS1 and PPARG. This evidence concerns the gene PPP1CA and metabolic dysfunction-associated steatotic liver disease.