DUX4 and facioscapulohumeral muscular dystrophy: To determine whether the expression changes identified in the RD cells ectopically expressing DUX4 can be detected in the FSHD myoblasts, we performed real-time qRT-PCR and validated the significant up-regulation of MYOD (19 fold, p<0.01), MYOG (110 fold, p<0.01), ZSCAN4 (32 fold, p<0.01), and UTS2 (229 fold, p<0.01) (Figure 4A–D) in immortalized FSHD cells as compared to the control immortalized myoblasts.