We found that levels of SFRP5 mRNA were significantly lower in AOM-induced tumors, compared with AOM-untreated normal colons (Figure 4A), P<0.01, whereas the levels of Cyclin D1 mRNA were markedly higher in AOM-induced tumors, relative to AOM-untreated normal colons (Figure 4C), P<0.05. This evidence concerns the gene CCND1 and infectious otitis media.