To determine the clinical significance of MACC1, the correlation between MACC1 overexpression and the clinicopathological features of HCC was investigated in a retrospective cohort of 308 HCC cases by IHC, including 26 cases of stage I (8.4%), 195 cases of stage II (63.3%), 61 cases of stage III (19.8%) and 26 cases of stage IV liver cancers (8.4%), based on the TNM staging. This evidence concerns the gene MACC1 and liver cancer.