Specifically, very-long-chain-specific acyl-coA dehydrogenase (ACADVL), actin-binding protein, anillin (ANLN), c-1-tetrahydrofolate synthase, cytoplasmic (MTHFD1), alpha-fetoprotein (AFP), and filamin-B (FLNB) increased in the healthy control versus before HCC treatment group and declined in the before HCC treatment versus after HCC treatment group. Here, AFP is linked to hepatocellular carcinoma.