The AUC values of 6 proteins exceeded 0.8–those of ANLN, BASP1, CAPN1 and PABPC1 were 0.920, 0.951, 0.946 and 0.949, respectively, in the healthy control versus before HCC treatment group, reflecting excellent specificity and sensitivity. The gene discussed is CAPN1; the disease is hepatocellular carcinoma.