Although the regulation of protein synthesis was identified as a valuable therapeutic target in treatment of MDD, one cannot rule out the possibility that fast-acting antidepressants affect trafficking and/or release of pre-synthesized BDNF from the brain cells; depressed patients report the alleviation of MDD symptoms within two hours of a single, low-dose intravenous infusion of the antidepressant drug [80]. This evidence concerns the gene BDNF and major depressive disorder.