GPT and cholestasis: Our previous reports have shown that rats treated once with ANIT (75 mg/kg, i.p.)have liver cell damage and cholestasis, judging from the serum levels of ALT and AST, indices of liver cell damage, and γ-GTP, total bilirubin, and total bile acid, indices of biliary cell damage and cholestasis, at 24 h, but not at 12 h, after the treatment [22–24, 37].