PIN1 and anaplastic astrocytoma: We also confirmed that Pin1 was expressed in both the cytoplasm and nuclei of glioma cells, which was consistent with Ryo et al(12) who reported that Pin1 expression was found to be confined to the nuclei in low grade astrocytoma at relatively low expression levels but exhibited enhanced expression in both the cytoplasm and nuclei of anaplastic astrocytoma and glioblastoma.