Recombined lentivirus expressing Med19 shRNA (si-Med19) or negative control shRNA (si-Negative) was transduced into human tongue cancer cells at the multiplicity of infection (MOI) of 100, and more than 90% of infected cells expressed GFP as determined with fluorescence microscopy 3 days post-transduction (Figure 1a). The gene discussed is MED19; the disease is infection.