Furthermore, the promoter methylation of RASSF1A in ESCC patients with the age group from 50–60 years old had a significantly higher risk to ESCC than in those with the age less than 50 years old (OR=3.11, 95% CI=1.10–8.73) (Table 3). Here, RASSF1 is linked to esophageal squamous cell carcinoma.