To determine the role of TβRII and consequently that of the TGF-β signaling pathway in regulating the malignant phenotypes of HCC cells, we knocked down TβRII in SNU423 and Sk-Hep-1 cells with the stable expression of a TβRII shRNA as described previously [22]. This evidence concerns the gene TGFBR2 and hepatocellular carcinoma.