We also observed the upregulation of glioma specific proteins [11] in the differentiating GIC03A and GIC03U, such as vimentin, VEGF, EGFR, MAPKs, KRAS, Musashi, FABP7, S100B, Cathepsin B, BAX, BAD, BID, CDK4, CDK6, as well as CD44, GFAP in proteomics or mRNA levels during 2 to 7 days of the serum stimulation of GICs (Table S2). This evidence concerns the gene CDK4 and central nervous system cancer.