In this paper, we demonstrate for the first time that the induction and interaction of integrin αV and FN, an ECM core protein, are key functions in the formation of a specific microenvironment, the so-called differentiation niche, by GICs, and that inhibitors such as blocking antibodies and RGD peptides coupled with anti-cancer drug might be useful for the abrogation of early events in GIC differentiation/proliferation and further glioma formation or propagation. The gene discussed is FN1; the disease is central nervous system cancer.