We also observed the upregulation of glioma specific proteins [11] in the differentiating GIC03A and GIC03U, such as vimentin, VEGF, EGFR, MAPKs, KRAS, Musashi, FABP7, S100B, Cathepsin B, BAX, BAD, BID, CDK4, CDK6, as well as CD44, GFAP in proteomics or mRNA levels during 2 to 7 days of the serum stimulation of GICs (Table S2). Here, CTSB is linked to central nervous system cancer.