Our informatics analysis of the gene content of the 16p13.11 region found evidence that several of the genes in the region, such as ABCC1, NOMO1, NTAN1, and PDXDC1 have expression during brain development—as does NDE1. These may be candidate genes for second-hit mutations in patients with deletions of 16p13.11 and more severe disorders of cortical connectivity such as autism, intellectual disability, and schizophrenia. Here, NDE1 is linked to autism.