We validated this assay on random primed RNA from breast cancer cell lines and normal human mammary epithelial cells (HMECs) (Supplementary Figure S6) confirming our earlier observations (Figure 1B) that increased antisense expression in breast cancer cell lines MCF-7 and HCC-1954 is associated with decreased TFPI-2 expression, proving its validity. This evidence concerns the gene TFPI2 and breast cancer.