PTEN and acute myeloid leukemia: In the case of PTEN, two samples (P2 and P6) had lower read numbers (Supplementary Figure S5c), suggesting that these two cases of AML may harbour deletions involving PTEN. To confirm this using our limited material, we looked at differential allelic read counts for six intronic single-nucleotide polymorphisms within the PTEN locus, using PCR amplification followed by sequencing on a MiSeq sequencer.