Microinjection and ingestion have proven effective approaches to deliver dsRNAs, small interference RNAs (siRNAs), or short hairpin RNAs (shRNAs) of target genes into insects, thereby enabling in vivo studies of gene functions in various systems, including BtSnap in silverleaf whitefly (Bemisia tabaci) [50], c002 in pea aphid (Acyrthosiphon pisum) [51] and Calreticulin in BPH (N. lugens) [52]. Here, CALR is linked to benign prostatic hyperplasia.