CD86 and infection: Flow cytometric analysis was carried out to detect the recruitment and activation of DCs and B cells in the draining lymph nodes and the blood at 3, 6 and 9 days post infection (dpi).As shown in Fig. 2A and 2C, significantly more DCs (CD86+ and CD11c+) and B cells (CD40+ and CD19+) were detected in the draining lymph nodes (inguinal) of mice immunized with LBNSE-Flagellin and LBNSE-GMCSF than in mice immunized with the parent virus LBNSE at 6 and 9 dpi.