To determine if expression of GMCSF or Flagellin has adverse effects in mice, four groups of 10 ICR mice(6 to 8 weeks old, female ) were injected with 1×107 FFU of LBNSE, LBNSE-GMCSF, LBNSE-Flagellin or DMEM (mock infection) by the i.c. route and their body weights were measured daily for 2 weeks. The gene discussed is CSF2; the disease is infection.