Notably, the protein expression levels of the genes exhibiting the least significant expression differences (that is, highest P values) in this study, RORA and CYP19A1, were found to be significantly different in our previous study in which 12 autism tissue samples were compared with 22 controls[12], thus emphasizing the need for an expanded investigation of RORA targets involving additional brain tissues from individuals with ASD and controls, including samples from different brain regions. This evidence concerns the gene RORA and autism.