Results were confirmed at the protein level by Western blot (Fig. 1b). The prostate cancer cells PC-3 had the highest expression of RANK, both at mRNA and protein level, followed by the bone-seeking cell line MDA-231BO2, a clone isolated from MDA-231 cells with increased avidity for bone in vivo[33]. The gene discussed is TNFRSF11A; the disease is prostate carcinoma.