In the present study, we confirmed the regulatory relationship between miR-124, a known tumor-suppressive miRNA, and an oncogene, iASPP. We provide evidence that miR-124 can inhibit CRC cell proliferation, at least in part by targeting iASPP. Ectopic overexpression of miR-124 downregulated iASPP protein expression and upregulated NF-κB protein expression. The gene discussed is NFKB1; the disease is colorectal carcinoma.