To investigate if miR-124 can regulate CRC cell viability by targeting iASPP and upregulating NF-κB, we transfected miR-124 lentivirus, control lentivirus, iASPP shRNA or shRNA-control into SW480 or HT29 cells, and performed the MTT assay. This evidence concerns the gene NFKB1 and colorectal carcinoma.