In summary, we identified that miR-124 inhibits CRC cell viability, proliferation, and colony formation through targeting iASPP; these effects were due, at least in part, to upregulation of NF-κB. The miR-124/iASPP axis identified in this study may play a vital role in regulating the proliferation of CRC cells and may provide a potential diagnostic and therapeutic target for CRC. This evidence concerns the gene PPP1R13L and colorectal carcinoma.