To determine whether the 3′-UTR of iASPP mRNA is a functional target of miR-214 in CRC cells, we created a WT-iASPP 3′ UTR luciferase reporter vector (WT-iASPP), as well as a MUT-iASPP 3′ UTR luciferase reporter vector (MUT-iASPP) by sequentially mutating the predicted 8-base pair miR-124 binding site in the iASPP 3′ UTR (Figure 2(a)). This evidence concerns the gene PPP1R13L and colorectal carcinoma.