All these host markers have previously been evaluated in QFT-IT supernatants and shown to be potentially useful for use singly or in combination with IFN-γ for diagnosing M.tb infection (especially IP-10 and TNF-α) [17], [18], [27], [50], [51], or for discriminating between TB disease and LTBI (IP-10, TNF-α, EGF, TGF-α, and VEGF) [16], [19], [20], [28]. Here, TNF is linked to tuberculosis.