Figure 2 shows the mRNA expression levels of UROtsa-3, UROtsa-4, the cervical carcinoma cell line HeLa, the urothelial carcinoma cell line T24, the papillary tumor cell line RT4, and a primary urothelial cell line (HUEPC) as determined by Real-Time PCR. Each cell line had a unique expression pattern. UROtsa-3 showed an mRNA profile that was markedly different from UROtsa-4 and HUEPC but resembled T24. KRT20 and UPK1A could only be detected in the well-differentiated papillary tumor cell line RT4. This evidence concerns the gene UPK1A and urothelial carcinoma.