ATAD2 and hepatocellular carcinoma: In order to further investigate immortality-associated expression of ATAD2 in HCC cells, we induced senescence arrest in Huh7 cells by 0.1 μM Adriamycin treatment (Fig. 8c) as previously described [40], and compared ATAD2 expression between Adriamycin-treated and control Huh7 cells by western blot assay.