To address this, we made use of U87 glioma parental cell line along with its two derivatives U87-wtEGFR (that stably overexpress 3′-UTR-less wild type EGFR) and U87-ΔEGFR (that stably overexpress 3′-UTR-less vIII variant of EGFR). Here, EGFR is linked to central nervous system cancer.