Finally, we analyzed miRNA-target associations at the mRNA level in ESCC and OSCC primary samples (Fig. S6A), however significant correlations were not found between miR-655 expression and expression of ZEB1 or TGFBR2 transcripts as well as a large number of miRNAs and their targets, indicating that not only miR-655 but also other unknown molecules including transcription factors might regulate ZEB1 and TGFBR2 expression. The gene discussed is ZEB1; the disease is esophageal squamous cell carcinoma.