These protective effects against NAFLD were also supported by significant decreases in plasma levels of ALT and AST in GPE + OFE-supplemented obese mice, since the elevated liver aminotransferase (ALT and AST) is positively correlated to 90% patients with nonalcoholic steatohepatitis, characterized by a liver lipid accumulation combined with hepatic inflammation [55]. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.