Mraovitch and Calando [73] used immunocytochemistry to determine the regional and temporal distribution of Fos protein expression in awake and unrestrained rats after a unilateral stereotaxic microinjection of the cholinergic agonist carbachol in the thalamic ventroposterolateral and reticular nuclei, previously shown to cause limbic and generalized seizures [73]. The gene discussed is FOS; the disease is Seizure.