In non-malignant lung tissues, only 2 cases of squamous metaplasia in non-tumor adjacent lung tissues were positive for Nanog (Figure 2F), whereas, Nanog staining was detected in 36 of 50 (72%) cases of lung cancer, in which 29 cases were diffusely positive, 6 cases were focally positive and 1 case was isolated positive (Figure 2F). The gene discussed is NANOG; the disease is neoplasm.