SACK1B and colorectal cancer: To confirm the requirement for FAM83B in mutant RAS-mediated tumorigenicity in vivo, we infected HCT116 colorectal cancer cells (KRAS-G13D and elevated FAM83B mRNA) with lentiviruses expressing shRNAs targeting FAM83B (B) or GFP (G), injected NOD/SCID mice, and measured tumor formation after two weeks.