However, since the VMH of germline SF-1 KO mice had disrupted cytoarchitecture, the obesity phenotype in the transplanted animals could be a consequence of impaired VMH formation rather than SF-1 absence in the nucleus (Majdic et al., 2002; Davis et al., 2004; Zhao et al., 2008). This evidence concerns the gene SF1 and obesity due to melanocortin 4 receptor deficiency.