Using various assays, we find that MA treatment led to the induction of apoptosis in breast cancer cells which was seen by enhanced loss of plasma membrane polarity as detected by Annexin V staining (Fig. 4), sub-G1 phase accumulation as indicated by FACS analysis (Fig. 3) and loss of mitochondrial transmembrane potential observed by JC1 staining (Fig. 5) and DNA fragmentation (data not shown). This evidence concerns the gene ANXA5 and breast carcinoma.