With previous reports that the transformed hepatoma cells have impaired double stranded RNA and virus-activated IFN responses [25]–[26], HepG2/C3A and A549 cells, which showed negative sense RNA at early time points were stimulated with poly (I∶C) (1–5 μg/ml) to check for their response. This evidence concerns the gene IFNA1 and hepatocellular carcinoma.