Overexpression of regucalcin rescues cell death and apoptosis induced with various factors (including TNF-α, TGF-β, LPS, insulin, IGF-I, Bay K 8644, PD98059, dibucaine, thapsigargin, or sulphoraphan), which those signaling mechanisms are different in the hepatoma cells and normal kidney cells. This evidence concerns the gene TNF and hepatocellular carcinoma.