Regarding post-mortem analyses (23 days after last bleomycin administration), only a sixfold administration of 1.0 mg/kg bleomycin induced an amount of lung fibrosis that led to a significant higher amount of collagen as detected by picrosirius staining (Figure 9B), hydroxyproline (Figure 9C), and Col1α1 gene expression (Figure 9D). The gene discussed is COL1A1; the disease is pulmonary fibrosis.