Finally, the siRNA of Gli3 that provoked an important diminution in Gli3 mRNA levels but not a decrease in Gli1 or Gli2 mRNA levels (Figure 1C), did not reduce A549 adenocarcinoma cell proliferation or cell viability (Figure 1D and E) and instead caused a slight increase in cell number of A549 adenocarcinoma cells (Figure 1D) along with an increase in Gli1 mRNA levels (Figure 1C). The gene discussed is GLI3; the disease is adenocarcinoma.