Wondering whether αvβ3 might play a role in BSP-induced breast cancer metastasis, we transfected the 231BO-BSP27 cells with a pIRES2-hBSP-EGFP construct or the pIRES2-EGFP control construct, followed by detection of the BSP, β3 and αvβ3 levels with western blot and flow cytometry. This evidence concerns the gene IBSP and breast cancer.