CD8A and neoplasm: Figure 4A shows a detectable, but sparse, distribution of Pmel-1-specific T cells inside the tumor mass. A single cell suspension was prepared from whole-brain and analyzed using quantitative FACS analysis methods [27]. In the brain cell suspension, Pmel-1-specific T cells could be detected by FACS (Fig. 4B–C), where the average number (n = 3) of cells was approximately 44,000 per brain (n = 3), and each brain contained two Gl261 tumors. In the control group, where wild-type CD8+ T cells were infused, significant T cell trafficking into the brain was not observed via FACS (Fig. 4D).