Our open questions were i. how does eNOS play a role in prostate cancer under basal conditions or in response to estrogens and, by extension, in the estrogen-dependent transcriptional program associated with prostate cancer aggressive phenotype?, and ii. Does the nuclear eNOS function involve molecular interactions with proteins able to modify the chromatin structure and alter the transcriptome in estrogen-responsive prostate cancer cells? The gene discussed is NOS3; the disease is prostate carcinoma.