To explore whether these splicing regulators are involved in exclusion or inclusion of exons 2 and 3, we used siRNA to deplete hnRNP A1/A2 or SF2/ASF in human NSCLC cells A549 and Calu-6 and performed semi-quantitative RT-PCR to analyze change of alternative splicing of IRF-3 using consensus primers located in exon 1 and exon 4. This evidence concerns the gene IRF3 and non-small cell lung carcinoma.