We have established that organ culture, like experimental stroke, increases cerebrovascular expression of contractile GPCRs such as 5-hydroxytryptamine type 1B (5-HT1B), angiotensin II (Ang II) type 1 (AT1) and endothelin (ET-1) type B (ETB) receptors in the vascular smooth muscle cells (VSMCs) [11]. The gene discussed is AGTR1; the disease is stroke disorder.