The discrepancy in treatment-induced effects of Tregs based on phenotype is not only seen in patients treated with ipilimumab – in a study evaluating melanoma patients receiving a dendritic cell vaccine along with IL-2, the authors observed that a decrease in CD4+CD25hi T cells correlated with a clinical response; however, they also found that there was no correlation between changes in CD4+CD25hiFoxp3+ T cells and disease stabilization (Berntsen et al., 2010). This evidence concerns the gene CD4 and melanoma.