Considering the necessity of using new molecular markers to accurately predict the malignance behavior of bladder tumors, we evaluated the prognostic value of Oct4 expression, as a well-known stem cell molecular marker, in FFPE samples of bladder tumor tissues by means of semi-quantitative RT-PCR and immunohistochemistry (IHC). The gene discussed is POU5F1; the disease is urinary bladder neoplasm.