Two CRC cell lines, HCT116 and RKO, were treated with an anti-VEGF mAb, which acts exclusively on secreted/extracellular VEGF, for 2 or 60 days (mAb-short or mAb-long cells, respectively), with non-immune control IgG for 2 or 60 days (IgG-short or IgG-long cells, respectively), or without any treatment (none). The gene discussed is VEGFA; the disease is colorectal carcinoma.