Owing to the role of Ngn3 in glucose homeostasis, and the association of obesity with endocrine disorders such as type 2 diabetes, we next investigated in Nkx2.1iCre/+;Ngn3flox/flox mice the glucose/insulin response system as well as a number of other hormones associated with obesity (in the same cohort of n=9 for each sex and genotype). This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.