PTGS2 and Familial prostate cancer: We first evaluated the pro-inflammatory environment in three androgen-independent human prostate cancer lines, namely, 1-LN, DU-145, and PC-3, by quantifying p-cPLA2Ser505, COX-2, EP2, EP4, and PGE2 in these cells stimulated with either buffer or 8-CPT-2Me-cAMP (Figure 1).