Comparing with recombinant MMP-12 which exhibited the highest activity in 5 mM CaCl2 (Figure 5), MMP-12 activity levels in induced ascending aorta specimen from 12 AD, 4 CAD, and serum samples from 15 AD and 10 healthy patients were assessed using a FRET assay. The gene discussed is MMP12; the disease is coronary artery disorder.