In order to assess the specificity of eIF5A1 over-expression for inducing apoptosis in cancer cells rather than non-malignant cells, A549 lung carcinoma cells and WI-38 normal lung fibroblast cells were analyzed for induction of apoptosis by Annexin/propidium iodide (PI) staining following infection of Ad-eIF5A1 or Ad-eIF5A1K50A (Figure 7A). Here, EIF5A is linked to cancer.