Ad-eIF5A1 and Ad-eIF5A1K50A infection of A549 cells did not deplete hypusine-eIF5A1 levels (Figure 2A), indicating that the consequences of eIF5A1 and eIF5A1K50A over-expression are due to accumulation of non-modified eIF5A1 and not to depletion of hypusine-eIF5A levels. This evidence concerns the gene EIF5A and infection.