For this reason, we also compared the levels of chymase in these two groups of primary and secondary infection and found that while there were no differences in the chymase levels in patients diagnosed with DF between primary and secondary infection groups (Figure 6E), interestingly, significantly higher chymase levels were detectable in DHF patients with secondary infection compared to DHF patients diagnosed during their primary infection (Figure 6F). This evidence concerns the gene CMA1 and Dengue hemorrhagic fever.