In contrast to transfection with control vector, transfection with CAS162 reporter significantly increased luciferase activity in several cancer cell lines: two malignant mesothelioma lines (MSTO, JMN); and two hepatocellular carcinoma cell lines (Li7, Kim1) (Fig. 5F, 5G; Fig. S8B), suggesting that CAS162 may contain the regulatory element for POLR2A gene expression. The gene discussed is POLR2A; the disease is cancer.