To further evaluate the influence of extracellular S100A8 or S100A9 on the viability and migration of CRC cells, we prepared recombinant proteins GST, GST-hS100A8 and GST-hS100A9 to treat cells, and GST was used as the control for GST-hS100A8 and GST-hS100A9. This evidence concerns the gene S100A8 and colorectal carcinoma.