In addition, our results showed that higher concentrations of recombinant S100A8 or S100A9 protein monomer (40-120 μg/ml) had no effect on the survival of HCT116 and SW480 cells, which is supported by previous studies showed similar effects on HepG2 cells, mammary carcinoma cells and cervical cancer cells [16], [21], [53]. The gene discussed is S100A8; the disease is breast carcinoma.